- Manas Fen Bilimleri Dergisi
- Vol: 1 Issue: 6
- Development of Nephelometric Α1-At Method and its Performance Characteristics
Development of Nephelometric Α1-At Method and its Performance Characteristics
Authors : M. Çalapoğlu, O. Değer, A. Alver, N. Ş. Çalapoğlu, E. Kalay, C. K. Buruk
Pages : 29-39
View : 12 | Download : 6
Publication Date : 2005-01-01
Article Type : Other
Abstract :α1-AT, a glycoprotein of molecular mass of 52 kDa, is a major plasma serine protease inhibitor serpin . α1-AT plays a central role as a protease inhibitor in controlling tissue degradation. As a major protease inhibitor in human plasma, α1-AT can complex with a broad spectrum of proteases including elastase, trypsin, chymotrypsin, thrombin, and bacterial leukocyte elastase. α1-AT is an acute phase reactant whose plasma concentration can rise by 3 to 4-fold above normal during inflammation, infection and malignant disease. α1-AT deficiency, one of the most common hereditary disorders, mainly affects the liver and lung [1,2]. The concentrations of α1-AT in plasma can be measured either by functional assays or by immunochemical methods. Functional assays evaluate inhibition of trypsin or elastase. Immunologic methods include radial immunodiffusion, electroimmunoassay and automated nephelometric methods.Keywords :