- KSÜ Doğa Bilimleri Dergisi
- Vol: 15 Issue: 3
- Verticillium dahliae ile İnokule Edilen Yonca Bitkisinden (Medicago sativa cv. Vertus) Elde Edilen F...
Verticillium dahliae ile İnokule Edilen Yonca Bitkisinden (Medicago sativa cv. Vertus) Elde Edilen Fenilalanin Amonia Lyaz Enziminin Kademeli Olarak Saflaştırılması
Authors : Murat Dikilitas
Pages : 19-22
Doi:10.18016/ksujns.60327
View : 10 | Download : 3
Publication Date : 2012-12-31
Article Type : Other
Abstract : ABSTRACT: An increased activity in phenylalanine ammonia lyase (EC 4.3.1.24, PAL) enzyme in lucerne seedlings ( Medicago sativa L. cv. Vertus) following inoculation with Verticillium dahliae was measured after 48 h of inoculation. Purifications of PAL with 0-50% (NH 4 ) 2 SO 4 precipitation and Sephadex G-100 gel filtration resulted in 1.75 and 1.97 fold increases with 75 and 60 % recoveries, respectively. This study showed that the purification of the enzyme clearly resulted in significant differences in respect to activities between fractions. This property could be important for further characterization of pathogen-related proteins.Keywords :