- İstanbul Üniversitesi Eczacılık Fakültesi Dergisi
- Vol: 45 Issue: 2
- Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using...
Physiological and genetic analysis of cellular sodium and lithium response/resistance behavior using the yeast Saccharomyces cerevisiae as a model organism
Authors : Ş. Hande Tekarslan 1, 2, 3, ceren Alkım 1, 2 Carola Hunte 4, Z. Petek Çakar1, 2
Pages : 165-179
View : 13 | Download : 7
Publication Date : 2016-11-22
Article Type : Research
Abstract :Abstract: The yeast Saccharomyces cerevisiae is a simple eukaryote and an excellent model organism for molecular biology. In this study, a NaCl-resistant S. cerevisiae mutant obtained by inverse metabolic engineering was used as a model to investigate responses and resistance behavior to NaCl, LiCl, KCl, TMA, spermine and sorbitol stresses., at physiological and genetic levels. The physiological spot test results revealed that the NaCl-resistant yeast mutant showed higher resistance to LiCl and NaCl. Gene expression analysis by qRT-PCR revealed that ENA6 and NHA1 genes of the mutant were induced in the absence and presence of LiCl and NaCl. The dysfunction of Na+/H+ antiporters are related to several diseases such as hypertension, epilepsy, postischemic myocardial arrhythmia, gastric and kidney disease, diarrhea, and glaucoma. Thus, the NaCl-resistant yeast mutant could be used to understand cellular sodium and lithium resistance mechanisms and the function of Na+/H+ antiporters also in higher eukaryotic organisms, including humans. Key words: Saccharomyces cerevisiae, stress resistance, salt-resistance , lithium resistance, NHA1Keywords :