- Atatürk Üniversitesi Ziraat Fakültesi Dergisi
- Vol: 8 Issue: 1
- ERZURUM KARS VE AĞRI İLLERİNDE Q HUMMASI ÜZERİNDE ÇALIŞMALAR
ERZURUM KARS VE AĞRI İLLERİNDE Q HUMMASI ÜZERİNDE ÇALIŞMALAR
Authors : Necdet Leloğlu
Pages : 0-0
View : 10 | Download : 4
Publication Date : 2010-12-28
Article Type : Research
Abstract :ÖZET Bu çalışmada Erzurum, Kars ve Ağrı illerini içine alan bölgede, insan, -koyun ve sığır serumlarında Q humması antikor durumu ve bu hastalığın insan ve hayvanlar arasında muhtemel yayılma yolları incelenmiştir. Bunun için ·semınlar mikroaglutinasyon metodu ile serolojik testlere tabi tutularak, antikor titreIeri tetkik edilmiştir. Koyun ve sığır serumlarının bir kısmı mikroaglutinasyon, kapilar tüp aglutinasyonu ve kompleman fik:zasyon metodları ile ayrı ayrı muayene edilerek; alınan sonuçlar karşılaştınlmıştır. Sewlojik testlerde pozitif sonuç veren bazı, koyun ve sığır senıınları kaboylara intraperitonal enjekte edilerek hastalığın etkeni olan Cox.iella burnetii'nin izolasyonuna çalışılmıştır. Ayrıca çalışma bölgesinde altı belirli yerde, koyunlardan top~ lanan Dermoserıtor cinsi kenelerden Coxiel/a burnetii'nin izole edilmesi denenmiştir. Oç ilden toplam olarak 456 koyun ve 262 sığır senımu incelenmi~tir. Koyun serumlarının %22, i inde, sığır serumlarının % 15,6 sında Q humması antikoruna raslanılmıştır. İncelenen 178 insan serumunda ise %11,2 oramnda Qbumması pozitif sonuç bulunmuştur. Deneme hayvanlarına enjekte edilen Q humması antikonı taşıyan serum1ardan, etken izolasyonu mümkün olmamıştır. Ancak, kene1er~ den Coxiella burnetii aynımıştır. SUMMARY STUDİES ON Q FEVER İN HUMAN, SHEEP AND CATILE IN ERZURUM, KARS AND AGRI PROVINCES A research was conducted to in vestigate the ex.istance of Q fever antibodies in human, sheep and cattle serums' by microagg1utination tests, and probaNe modes of transmission among humen and livestock in Erzurum, Kars and Ağrı provinces, in Eastem Turkey. In all the tests microagglutination metbodwas used as standart, in addition two other methods namely, capilrary . tube agglutination and complemant fixation were a1so used for some of the serums, and three methods were compared witb one another. In oder to isoIate the microorganism . (C.burnetii), some sheep and cattle serums which yielded positive in serological tests, were inoc.ulated to guinea pigs intraperitoneally. In addition to these for the purpose of isolation of C. burnetii, some ticks be10nging to Dermocentor genus were taken from the sheep, reprasenting the six localities in the region. These ticks were inoculated into guinea pigs intraperİtoneally, The anİmal serums were collected from Meat and Fish Company, and the Municipal Slougbterhouse of Erzurum, The human serums were obtained from Biöchemical Laboratories of Erzurum Numune HospitaI. The antİgens used for the sorological reactions were supplied by Instiutude Pasteur of France. 209 sheep and 108 cattle serums, representing Erzurum area, were examİned and among thern 50 e{23,4) sheep and 13 (% 12,0) cattle serums were found to have Q fever amibodies. 30 (%20,7) out of 146 sheep serums and 18 (%17, i) out of 105 cattle serums from Kars area gave posİtive results of Q fever. 21 (%20,6) out of 102 sheep and also LO (%20,4) out of 49 cattle serums were found to be positivc from Ağrı province. Of the total 456 sheep and 262 cettle serums from the three provinces were tested, and in ım (%22,1) sheep and 41 (%15,6) cattle serums, Q fever was ob served as positive. 178 human serums also were tested and 20 (% 11,2) out of them were found to carry Qfever antibodies. In order to compare the three different sero10gical methods, 204 sheep and cattle serums were examined and the results are as fol1ows: 39 (%19,1) serums positive with microagglutin ation, 34 (%16,7) and 36 (%17,6) serums positive with capillary tube agglutination and complament fixation ınethods recpectively. On the isolation of C. hurnetii, 38 sheep and cattle serums containing Q fever antbodies were injected into guinea pigs intraperitoneally. No clinical symptoms appeared in these animals during the first one month of the inoculation, but serological tests which were made 30 days later, indicated posttive reactions at the high level in the three guinea pigs. However the microscobic exaıninations failed to show the microorganisms in the blood samples of the animals. For iso1ating the agent, suspcnsions from Dermocentor ticks were injected into guinea pigs. During the first month of injections, the animals showed no clinical symptoms, but serological tests gave positive results in one guinea pig. The smears prepared from the blood and spleen of this animal showed rickettsia. T,he rickettsia taken from the blood and s~leen of the animal we,:e isolated in the yolk-sac cu1tures. In addition, social and geographica1 condit ons of the region were discussed as factors effecting the transmİssion of Q fever.Keywords :