Effect of Cryopreservation on DNA Damage and Various Sperm Parameters in the Post-Mortem Obtained Buffalo Sperm

Authors : Eser Akal, Murat Selcuk

Pages : 432-438

Doi:10.30607/kvj.1343776

View : 43 | Download : 97

Publication Date : 2023-09-30

Article Type : Research

Abstract :This study aimed to investigate the effects of freezing on DNA damage and some sperm parameters in epididymal sperm obtained from the buffalo testicles after slaughtering. Epididymal semen examination of 50 male Anatolian buffaloes (three years old or older) was performed after being obtained from slaughterhouses. Sperm progressive motility (%), viability (%), abnormal sperm (%), and sperm DNA damage (%) were analyzed. The study found significant differences (p<0.001) between fresh and thawed semen on the progressive motility, viability, and mid-piece, tail, and total abnormality rates. It was determined that the difference between the DNA damage values in fresh and thawed semen was significant (p<0.0001). Progressive motility rates of fresh and thawed semen were consistent with the studies conducted. It is thought that epididymal buffalo semen obtained post-mortem can be used in biotechnological methods. This research will contribute to further studies to increase the Anatolian buffalo population. More comprehensive studies should be performed to determine the impact of fertility due to the semen DNA damage and minimize the DNA damage in the freezing-thawing semen samples.
Keywords : Anadolu mandası, DNA hasarı, Epididimal, Sperma dondurma