Effects of Tarantula cubensis Extract on Experimental Acute Spinal Cord Injury in Rats

Authors : Serdar Aktaş, Zahir Kizilay, Nesibe Kahraman Çetin, Serap Ünübol Aypak, Murat Boyacioğlu, Adem Yavaş

Pages : 269-276

View : 132 | Download : 183

Publication Date : 2023-09-30

Article Type : Research

Abstract :Spinal cord injury (SCI) is a critical health problem that occurs after spinal trauma. In this study, the possible effects of Tarantula cubensis extract in the acute period were evaluated. For the rat trauma model, 28 Sprague dawley rats were used. All rats were divided into 4 groups (n=7); Control, injury (IG), prednisolone treatment (PG), and Tarantula cubensis extract treatment (TCG). Laminectomy was applied to all rats except the control group, and an aneurysm clamp was applied to the spinal cord for 60 seconds. BBB (Basso, Beattie, Bresnahan Locomotor Rating Scale) for clinical improvement assessment, histopathological (inflammatory response, neuron viability), and the amount of biochemical parameters 8-hydroxydeoxyguanosine (8-OHdG), myeloperoxidase (MPO), transforming growth factor beta 1 (TGF β-1), tumor necrosis factor alpha (TNF-α), interleukin 2 (IL-2) were evaluated. According to histopathological evaluations; less damage and inflammation were observed in the TCG group compared to the IG group. There was a statistical similarity between TCG and PG (p=0.005) BBB score TCG values were higher than PG and IG rats. MPO level was found to be significantly lower than TCG group IG and PG in laminectomy rats (p=0.001). TGF β-1 (ng-l), which is an anti-inflammatory cytokine, was measured at the highest level of TCG (p=0.001). TNF-α (ng-l) levels were higher in TCG, PG, and IG compared to the control group. IL-2 (ng-l) levels were decreased in TCG, PG, and control groups compared to the IG group (p=0.05). Tarantula cubensis extract has been determined neuroprotective effect by showing anti-inflammatory activity.
Keywords : Antiinflamatuar etki, Nöroproteksiyon, Omurilik yaralanması, Tarantula cubensis ekstraktı