Updated molecular characterization of Orf virus in Türkiye

Authors : Murat Kaplan, Bahattin Taylan Koç, Kemal Pekmez, Abdurrahman Anıl Çağirgan, Fatih Arslan

Pages : 375-382

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Publication Date : 2023-09-30

Article Type : Research

Abstract :Orf (ORFV) virus is endemic in sheep and goats in Türkiye and detected with increasing incidence in Spring. Despite this significant prevalence rate of ORFV, a few molecular characterization studies have been conducted in Türkiye and all of them have focused on only B2L gene region so far. The aim of this study was to determine the molecular characterization of different gene regions of ORFV isolated from field studies. In the study, partial genome sequencing of different gene regions (B2L, F1L and VIR) of positive ORFV isolates detected by real-time PCR from seven sheep with suspected Orf in 2018-2020 were performed. Molecular characterizations were determined by bioinformatics studies. After the molecular and in-silico processes, we found remarkable diversification in the evaluation of each gene region. This result showed that different variants are more likely to have circulated in different parts of the Türkiye. When the study is evaluated as a whole, it is thought that the F1L gene region should be considered in addition to the B2L gene region in molecular studies. Molecular data of ORFV should be updated and followed to provide the best efficiency of the prevention and control strategy.
Keywords : Amino asit, filogeni, moleküler, orf virus, Türkiye