Cryopreservation of Ram Semen Using Capsaicin Supplemented Tris Extender

Authors : Muhammed Enes Inanç, Fatih Avdatek, Şükrü Güngör, Mehmet Fuat Gülhan, Kemal Tuna Olğaç, Barış Denk, Deniz Yeni, Umut Taşdemir

Pages : 166-173

Doi:10.30607/kvj.1257228

View : 83 | Download : 136

Publication Date : 2023-06-30

Article Type : Research Article

Abstract :In this study, it was designed to reveal the effects of capsaicin on oxidative stress and freezability of ram semen. Ejaculates were taken from Sönmez rams and divided into five specimens and diluted with extender at different rates (4 mM, 2 mM, 1 mM, 500 µM) with and without capsaicin (control; C). Semen samples were thawed with a 37°C water bath for 30 seconds for post-thawed analysis. At the end of the study, sperm motility and kinetic parameters, plasma membrane acrosome integrity (PMAI), mitochondrial membrane potential (MMP), DNA damage, oxidant and antioxidant parameters were analyzed. A decrease was observed in the groups containing capsaicin compared to the C in terms of progressive, total motility and kinetic parameters (p<0.05). Besides, positive results were not obtained DNA integrity, PMAI and MMP (p<0.05). In conclusion; it was determined that capsaicin added to Tris extender did not have a positive effect on oxidative stress and freezing of ram semen.
Keywords : Spermatozaa, kapsaisin, koç, antioksidan