Expression Patterns of Some Lipogenic Genes and Fatty Acid Profile of Liver in Food-Restricted Rats

Authors : Hüseyin Özkan, Irem Karaaslan, Ufuk Kaya, Sevda Dalkiran, Ibrahim Alakuş, Baran Çamdeviren, Hasan Hüseyin Keçeli, Akın Yakan

Pages : 131-142

Doi:10.30607/kvj.1173180

View : 106 | Download : 152

Publication Date : 2023-06-30

Article Type : Research Article

Abstract :This study aimed to determine food restriction effects on the profile of fatty acids and major genes on lipogenesis expressions in liver. 16 Wistar albino rats were divided into two groups and different diets were given to groups for 4-weeks. First group was fed ad libitum (Control group), another group was fed the half amount of the daily requirement (Food Restriction group, FR). As well as weekly food consumption and body weight changes, total cholesterol, HDL, LDL, and triglyceride levels were determined at the end of the feeding period. In addition to the fatty acid profile, FASN and SCD-1 genes expression levels were measured in the liver. While the body weight averages decreased after 7 days and remained similar, plasma glucose levels were found lower in the FR. FASN was upregulated approximately 6 folds, and SCD-1 increased insignificantly about 3 folds in the FR. C15:0, C18:1 n9 trans, C18:2 n6 cis, C21:0, C20:2, C20:5 n3, n6 and UFA were lower, while C16:0, C18:2 n6 trans, C20:3 n6, C22:6 n3, C22:1 n9, C22:2 and SFA were higher in FR. In addition to considering the exposure time and rate of food restriction, molecular activity and interactions in other metabolic organs should be investigated.
Keywords : FASN, karaciğer, SCD-1, yağ asitleri, yem kısıtlama