Botrytis fabae and Bean common mosaic virus (BCMV) are the most common diseases of Faba bean (Vicia faba L.) in TRNC

Authors : Ayşegül Çolak Ateş, Hakan Fidan, Nihat Yilmaz, Ayda Konuksal

Pages : 115-122

Doi:10.29278/azd.371067

View : 77 | Download : 12

Publication Date : 2017-12-30

Article Type : Research

Abstract :Surveys were conducted on the fields of faba bean culture in Turkish republic of North Cyprus (TRNC) during 2011-2013 indicated that Botrytis fabae and Bean common mosaic virus (BCMV) are the diseases that cause crop loss at economic level. During the field surveys 117 infected plants that have the symptoms of both diseases were collected from 38 different locations. Symptomatologic, morphologic and classic tests indicated that 96 of the faba bean samples had Chocolate spot disease caused by Botrytis fabae and the conidia lengths have been measured between 12.7-22.4 x 9.4-17.8 microns. Faba bean samples with virus symptoms that were collected from 38 locations. All samples were infected by Bean common mosaic potyvirus (BCMV) serologically (DAS-ELISA). The 850 bp section of BCMV isolates coat protein was increased using RT-PCR method. This section was sequenced directly using ClonJet and characterization was determined. According to Blast obtained from sequences 98% and 96 % resemblances with Mexican and Columbian isolates have been found.
Keywords : Faba bean, Botrytis fabae, Bean common mosaic virüs, PCR