Biochemical Responses of Sainfoin (Onobrychis viciifolia Scop.) Callus Tissue to Low Dose Gamma Radiation

Authors : Ramazan Beyaz

Pages : 21-30

View : 24 | Download : 14

Publication Date : 2021-10-29

Article Type : Research

Abstract :In this study, biochemical changes in callus tissue caused by low dose (50 Gray) gamma radiation were investigated. For this purpose, seedlings were developed in vitro from seeds exposed to gamma radiation. The petioles of these seedlings were used as explants for callus formation. Biochemical parameters such as antioxidative enzyme activities (superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR)), malondialdehyde (MDA) and proline content were measured in 30-day callus tissue. As a result of the study, it was determined that the activities of antioxidative enzymes (except for APX), malondialdehyde and proline content were statistically increased in callus tissues. Considering the increase rate in callus tissues, it was determined that the highest increase in biochemical parameters was in SOD activity and proline content. The importance of this study is to show that irradiation of seeds with gamma radiation is sufficient to change the biochemical content of callus tissues obtained from any plant part under in vitro conditions. As a result, a plant breeders who uses biotechnological approaches in their studies can easily benefit from the evidence presented in this study.
Keywords : Gamma radiation, Antioxidant enzymes, Malondialdehyde (MDA), Proline