Molecular investigation of the relationship between vector tick and host in Lumpy Skin Disease

Authors : Hamza Kadi, Cüneyt Tamer, Emre Ozan, Mitat Kurt, Selma Kaya, Abdullah Cavunt, Harun Albayrak

Pages : 11-15

Doi:10.35864/evmd.1244360

View : 38 | Download : 44

Publication Date : 2023-06-20

Article Type : Research Article

Abstract :Lumpy Skin Diseases Virus (LSDV), belonging to the capripoxvirus genus of the Poxviridae family, causes significant infections in cattle, especially in African and Middle East countries. LSD is an arboviral disease that spreads with bloodsucking flies as the primary source of infection. However, in experimental studies, nucleic acid belonging to the LSDV has been detected in ticks, and it has been reported that ticks may also play a role in transmission. Within the scope of this study, we have aimed to investigate the vector tick-host relationship of the disease by collecting blood and tick samples from cattle, sheep, and goats in Samsun, Sivas, and Tokat provinces, where LSD infections are intensely detected in the Black Sea Region in Turkey. For this purpose, ticks and blood samples were collected from 88 cattle, 511 sheep, and 108 goats with tick infestation between March 2016 and October 2017. A total of 2508 ticks were collected from these animals, whose blood samples with EDTA were taken, and the ticks were classified according to species. Blood samples and ticks were tested to reveal the presence of LSDV nucleic acids by real-time PCR, and LSDV nucleic acids could not be detected in both blood samples or ticks.
Keywords : SNEHV, Kene, Vektör, real time PCR