Ependimomalarda Antiapoptik BCL-2 Protein ve Tümör Supressör P53 Protein İfadesi ve Proliperatif İndeks Kİ-67 ile Histolojik Tipler ve Derecelerin İlişkisi

Authors : Serdar Altinay, Aydın Sav, Özlem Yapicier, Ilhan Elmaci

Pages : 232-241

View : 56 | Download : 13

Publication Date : 2012-12-01

Article Type : Research

Abstract :Background: The objectives of this study are to investigate both proliferative activity of ependymomas as determined by MIB-1 also known as Ki67 labeling index LI and p53 and bcl-2 proteins by immunohistochemical analysis to assess their expressivity and to correlate findings with ependymoma subtypes and tumor grades. Methods: We investigated routinely formalin-fixed, paraffin-embedded brain and spinal cord biopsy tissues from 33 patients with ependymal neoplasms retrieved from the files of the Neuropathology division, Institute of Neurological Sciences, Marmara University. This study covers 33 tumors with the histological features of 2 subependymomas, 3 myxopapillary ependymomas Grade I , 13 ependymomas Grade II , and 15 anaplastic ependymomas Grade III . Results: Grade I and Grade II ependymomas showed low proliferative activity. P values were significant for atypia p=0.00034, for necrosis p=0.0011, for mitosis p=0.00447, for density p=0.0000. MIB-1 immunexpressivities were seen in 78% of anaplastic ependymomas. Protein expression of bcl-2 was detected in all of ependymoma subtypes and grade, specifically prominent in myxopapillary ependymomas. Seven tumors %21.2 showed nuclear p53 positivity with different percentages with staining of nuclei. Positivity was significant in anaplastic ependymomas %33.4 . We found LI values with significant correlation between supratentorial and infratentorial ependymomas Mann-Whitney-U test, p=0.012 Ki-67 LI values and patients with >18 years old and
Keywords : ependymoma, p53 gene, MIB-1, bcl-2