Elektrikle Uyarılmanın ve K+ Depolarizasyonunun Sıçan Striatal Dilimlerinde Asetilkolin ve Kolin Salıverilmesine ve Doku Düzeylerine Etkisi

Authors : Ismail Hakkı Ulus

Pages : 80-87

View : 47 | Download : 14

Publication Date : 2011-06-01

Article Type : Research

Abstract :Effects of electrical stimulation and potassium depolarization on acetylcholine and choline release and synthesis in rat brain striatal slices Objective: The aim of the study was to determine effects of electrical stimulation and potassium depolarization on acetylcholine and choline release and synthesis in rat brain striatal slices Methods: Striatal slices from rat brain were perfused with physiological medium under basal and stimulated conditions, either with electrically or high potassium medium, for 120 min. Acetylcholine and choline contents of the perfusate and tissue were assayed radioenzymatically. Results: Striatal slices released 376±20 pmol/mg protein/120 min acetylcholine and 2375±85 pmol/mg protein/120 min choline into the perfusate at rest. When sliced stimulated by high potassium or electrically, acetylcholine release into the perfusate were increased by 5-6 folds and reached to 2360±85 or 2830±174 pmol/mg protein/120 min, respectively. Choline release into perfusate was not change during electrical stimulation. During high potassium depolarization, choline release increased by 50% at first 20-min period but decreased by 25% during the whole of 120-min period. The tissue contents of acetylcholine, but not choline, decreased slightly by 20%; p
Keywords : striatal slices, acetlcholine, choline, eletrical stimulation, K+ depolarization