Genetik Absans Epilepsili Sıçanların GAERS Hipokampusunda Glukoz–6-Fosfataz’ın Histokimyasal Olarak Dağılımı

Authors : Gözde Erkanlı Şentürk, Şükrü Midillioğlu, Şehnaz Bolkent, Serap Arbak

Pages : 26-30

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Publication Date : 2011-03-01

Article Type : Research

Abstract :Epilepsy is a disease with repeated seizures and usually caused transient ischemic attacks. Genetic Absence Epilepsy Rats from Strasbourg GAERS were recently used as a model for absence epilepsy. It has been used in experimental research in order to form the human absence epilepsy model since 1980. The aim of this study is to reveal changes in glucose–6–phosphatase activity which is the key enzyme of carbohydrate mechanism, at light microscopical levels by means of histochemical methods in GAERS’s brain. The correlation between the glucose metabolism and epileptogenesis has also been tried to be evaluated in this study. In control groups, non-epileptic Wistar albino, 4-months old, male, 220–240 gr rats were used n: 4 . Six-months old, 250–300 gr GAERS rats with previously determined absence seizures constituted the experimental group n: 4 Glucose–6-phosphatase histochemistry were examined within hippocampal Dentate Gyrus DG and Cornu Ammonis CA regions. Glucose–6–phosphatase reactivity were determined to be increased in DG and CA1 region in GAERS groups compared to the control group. As a result, based on the knowing generalized convulsive seizures increases the glucose utilization, glucose–6–phosphatase may be given in experimental studies, in order to treat with glucose–6–phosphatase in the cases of epilepsy in the future
Keywords : glucose-6-phosphatase, GAERS, histochemistry