Bacillus aryabhattai SMNCH17-07 Strain: First Isolation and Characterization from Textile Waste Water with Evaluation of Its Decolorization Ability against Azo Dyes

Authors : Safiye Elif Korcan, Kübra Çitekci, Büşra Aydin, Ahmed Badri Abed, Gülderen Uysal Akkuş

Pages : 99-107

Doi:10.32710/tekstilvekonfeksiyon.929205

View : 29 | Download : 11

Publication Date : 2022-06-30

Article Type : Research

Abstract :Azo dyes are largely entered into various industrial products. Conventional treatment techniques are insufficient to eliminate azo dyes entirely from effluents due to the ability of these dyes to maintain color, steadiness and being difficult to transformation. Bioremediation procedures have -been proved as a promising method to remove azo dyes effectively, due to their advantages such as the low cost, being environmentally safe and the large scale usage capability. In this study, SA3 bacterial isolate, has been collected from textile waste water area in industrial zone located in Usak province, Turkey. Phenotypic identification and phylogenetic determination on the basis of partial 16S rDNA sequence comparisons indicated that this strain is 100 % Bacillus aryabhattai SMNCH17-07 strain. SA3 isolate was Gram positive, central spore-forming and rod-shape bacteria .The isolate gave positive results for catalase, NaCl, Voges-Proskauer, starch hydrolysis assays and negative results for the citrate reaction. The decolorizing ability of this strain was evaluated against CI Acid Blue 193 CI 15707 and CI Acid Red 88 CI 15620 dyes. The results showed that absorbance rate of Acid Blue by this isolate was (33, 17%) after 216 hours. While Absorbance rate of Acid red by this isolate was (62,68 %) after120 hours. According to FTIR spectrometer results of dyes adsorption it was found that bacterial retention sites were possibly aromatic and aliphatic (C = C, C = N, N = N) as well C‐O groups. As the first report on the isolation of Bacillus aryabhattai SMNCH17-07 strain from textile waste water with the evaluation of its ability to remove azo dyes, we suggest testing this bacterium as a low cost and ecofriendly bioremediator agent against further harmful dyes and pollutants.
Keywords : Bacillus aryabhattai, , decolorization, acid red, acidblue