Seroprevalence of Galactomannan Antigen in Erzurum and Comparison of Two Different Test Kits for Galactomannan Detection

Authors : Demet Çelebi, Ozgur Celebi

Pages : 6-11

View : 23 | Download : 8

Publication Date : 2022-01-15

Article Type : Research

Abstract :ABSTRACT: Background In this study, we aimed to determine the prevalence of galactomannan antigen in various risk groups and to compare the results of two different test kits used in diagnosis and to determine false positivity rates. Methods Bio-Rad Platelia Aspergillus Ag kit was used to detect Aspergillus galactomannan antigen in serum samples of patients who were hospitalized in various clinics or admitted to hospital for serious diseases. In order to detect false positives, some of the samples found positive in this test were studied with Bio Bio-Rad” kits as well as "Dynamiker Biotechnology (Tianjin) DNK-SM-1402-1” test kits for the second time. The same procedure was repeated for the third and fourth times. Results Galactomannan antigen was searched in 735 different cases. In 306 (41.6) cases were obtained in at least one positive result study. Galactomannan antigen was the most common in septicaemia (75.0%); the lowest rate was found in patients with pre-diagnosis of neoplasm (21.8%). Galactomannan antigen positivity was highest in patients over the age of 65. Galactomannan antigen positivity was found to be very similar in the second and third studies of positive samples. In the fourth repetition, both firms gave 100% similar results. From the first to the last, GM positivity rates gradually decreased and GM positivity of 98 (32.0%) out of 306 positive cases in the first study has continued. Conclusion It was determined that the kits belonging to two companies can be used with the same reliability and the positive rates of both tests gradually decreased.
Keywords : aspergillosis, anemia, Galactomannan Antigen, Lymphoma, Leukemia, Septicemia