Cytoxic Effect Levels Of Sheep Whey Protein in Colorectal Adenocarcinoma Cell Line (Caco-2)

Authors : Ilhan Sabancilar

Pages : 24-29

Doi:10.56484/iamr.1162647

View : 23 | Download : 14

Publication Date : 2022-12-29

Article Type : Research

Abstract :Abstract Objective: It was aimed to determine the antitumor and antiproliferative activity of whey protein (whey) obtained from sheep colostrum isolation on colorectal cancer cells (Caco-2). Method: Colostrum was separated into fractions as whey proteins using the isoelectric point. Before cell culture was studied, whey protein was sterilized by a membrane filter. Antitumoral activity levels of different concentrations of lyophilized proteins were measured by MTT viability test on cells and IC50 values were determined. Results: Sheep whey proteins were incubated with Caco-2 cells for 24, 48 and 72 hours with serial dilutions starting with 3200 µg/ml and decreasing. When incubated with sheep whey Caco-2 cells, cell viability: 66.34% at 24 h; IC50 value of 8.414 µg/ml; IC50 value of 46.61% at 48 h was 5,838 µg/ml; The IC50 value of 4.61% at 72 h was found to be 4,741 µg/ml. Conclusion: It was concluded that whey proteins obtained from sheep colostrum isolation have apoptotic and anticarcinogenic effects on Caco-2 cancer cell line and significantly inhibit the growth of tumor cells. We think that the results of this study will lead to studies to be carried out in vitro.
Keywords : colorectal adenocarcinoma, cytotoxicity, whey proteins, Colostrum