Synthesis of Nanoparticles Loading Indenopyrazole Derivatives and Evaluation of Biological Features

Authors : Murat Doğan, Ümit Muhammet Koçyiğit, Hayreddin Gezegen

Pages : 830-834

Doi:10.33808/clinexphealthsci.1055921

View : 16 | Download : 10

Publication Date : 2022-12-30

Article Type : Research

Abstract :Objective: In this study, it was aimed to prepare nanoparticle formulations using chitosan, a cationic natural polymer, and tripoly phosphate, and to perform mechanical characterization and in vitro cell culture studies. In addition, the cytotoxic effects of nanoparticles containing indenopyrazol derivatives against human glioma cells (C6) and human cervical cancer cells (HeLa) were investigated. Methods: Within the scope of the study, nanoparticles containing indenopyrazole derivative were prepared and characterization of particle size, zeta potential and morphological properties were performed. XTT cytotoxicity test was applied to evaluate the antiproliferative activities of nanoparticles containing these components. Results: Particle size, zeta potential and morphological properties of nanoparticles were observed to be suitable for application. In vitro cell culture studies showed that nanoparticles containing indenopyrazol derivatives showed better cytotoxic effects in both cell lines. Conclusion: The results showed that the mechanical properties of nanoparticles containing indenopyrazol derivatives are suitable and can be applied in anticancer activity studies.
Keywords : Chitosan, Indenopyrazole derivatives, Nanoparticle, Antiproliferative activity