Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)

Authors : Hüseyin Arikan, Bayram Göçmen, Naşit Iğci, Bahadır Akman

Pages : 216-221

Doi:10.3906/sag-1211-11

View : 21 | Download : 10

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract : Atatür (1979) as previously described in detail (Arıkan et al., 2006). Briefly, venom samples were loaded onto the polyacrylamide gels and separated using a Canalco model 1200 electrophoresis apparatus. Gels were stained with 0.5% Amido black to visualize the protein bands and densitometric curves were obtained using a Gelman ACD-15 model 39430 densitometer at 500 nm.The authors received ethical permission for venom sampling (Ege University Animal Experiments Ethics Committee, 2010-43) and special permission for field studies (from Republic of Turkey Ministry of Forestry and Water Affairs).3. ResultsThe venom extracts of Vipera kaznakovi and Vipera ammodytes specimens had a viscosity higher than that of water. Although venom secretions of 2 specimens of V. kaznakovi (16.5 and 30 cm total lengths) were colorless, they were light yellow in the 55-cm-long specimen. In contrast, the venom extracts of 3 specimens of V. ammodytes were light yellow.The gel photographs of the venom protein samples of V. kaznakovi specimens are presented in Figure 1. According to our results, the venom proteins of V. kaznakovi were separated into 12 fractions or fraction groups in the 2 shorter individuals (16.5 and 30 cm), while there were 13 fractions or fraction groups in the gel of the 55-cm-long individual (the longest one). Our comparative results of electrophoretic density curves (protein fractions) revealed significant quantitative differences especially between the 2 shorter individuals, while similar electrophoretic mobility patterns and fraction numbers were observed (Figures 1–4). 
Keywords : Vipera ammodytes, polyacrylamide disc gel electrophoresis, densitometry