Sensitive determination of hydrogen peroxide in real water samples by high spin peroxo complex

Authors : Tuğba Yavuz, Levent Pelit

Pages : 435-447

Doi:10.3906/kim-1909-10

View : 20 | Download : 9

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :In this paper, a fast, cheap, simple, sensitive and selective spectrophotometric method based on high spin peroxo-Fe III -EDTAcomplexinthealkalinemediumwasdevelopedforthedeterminationofhydrogenperoxide H$_{2}$O$_{2}$ in real water samples. The purple-coloured complex with a maximum absorbance at a wavelength of 525 nm was formed. Various parameters such as type of stabilizer reagent and its concentration, reaction time, Fe III , EDTA and NH3 concentration were optimized. The method was confirmed with the Beer?s law with a molar absorption coefficient of267.36Lmol$^{-1}$ cm$^{-1}$ in the 8.3×10$^{-6}$ -4.08×10$^{3}$ mol/L concentrationrange. Sandell's sensitivityoftheproposed method was also calculated as 0.188 µg/cm2. LOD and LOQ were determined as 2.5 ×10$^{-6}$ and 8.3 ×10$^{-6}$ mol/L, respectively. Intraday and interday relative standard deviation of the proposed method for 2.0 × 10$^{-4}$ mol / L of H2O2 were found as 1.5% and 6.1%, respectively. The developed method is suitable for fast monitoring of H$_{2}$O$_{2}$ in different types of aqueous water samples without any sample preparation steps and acceptable recovery values between 90% and 118% were obtained. In the sample analysis, H$_{2}$O$_{2}$ removed solutions from the real water samples were used for blank correction in their analysis and this process provides more reliable and accurate results in real sample analysis.
Keywords : Hydrogen peroxide determination, high spin peroxo complex, spectrophotometry, real water samples