Development of a molecularly imprinted polymer-based electrochemical sensor for tyrosinase

Authors : Aysu Yarman

Pages : 346-354

View : 11 | Download : 8

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :For the first time a molecularly imprinted polymer (MIP)-based sensor for tyrosinase is described. This sensor is based on the electropolymerization of scopoletin or o-phenylenediamine in the presence of tyrosinase from mushrooms, which has a high homology to the human enzyme. The template was removed either by treatment with proteinase K or by alkaline treatment. The measuring signal was generated either by measuring the formation of a product by the target enzyme or by evaluation of the permeability of the redox marker ferricyanide. The o-phenylenediamine-based MIP sensor has a linear measuring range up to 50 nM of tyrosinase with a limit of detection of 3.97 nM (R$^{2}$ = 0.994) and shows good discrimination towards other proteins, e.g., bovine serum albumin and cytochrome c.
Keywords : Molecularly imprinted polymers, biomimetic sensors, tyrosinase, electropolymerization, scopoletin, o-phenylenediamine