Spectroscopic and thermodynamic approach to the interaction of nonperipherally substituted cationic phthalocyanines with calf thymus (CT)-DNA

Authors : Sevgican Tuncer, Ibrahim Özçeşmeci, Behice Şebnem Sesalan, Ayfer Kalkan Burat

Pages : 274-290

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Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :Novel, nonperipherally tetrasubstituted cationic metal-free and metallophthalocyanines (Zn, In) were synthesized in the present study. The binding constants, the disappearance of quenching effect of all cationic phthalocyanines on the fluorescence intensity of SYBR Green--disodium salt of deoxyribonucleic acid (DNA) from calf thymus complex, and the changes in T$_{m}$ of double helix DNA with thermal denaturation profile were investigated by UV-Vis and fluorescence spectrophotometric methods. To investigate the spontaneity of the reactions between DNA and novel quaternized phthalocyanines in buffer, thermodynamic parameters were employed.
Keywords : Calf thymus (CT)-DNA, morpholine, quaternized phthalocyanines, Gibbs, entropy