Determination of Hydrochlorothiazide and Enalapril Maleate in Tablet Formulations by Reversed-Phase HPLC

Authors : Idress F. Al-momani

Pages : 49-54

View : 14 | Download : 12

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :A high-performance liquid chromatographic procedure is presented for the determination of hydrochlorothiazide (HCT) and enalapril maleate (EM) in pharmaceutical tablets. An aliquot of the sample is dissolved in 15% acetonitrile (ACN) containing thiophylline as an internal standard and chromatographed on a Supelcosil LC-8 (5 m m), (150 mm x 4.6 mm i.d.) column. The mobile phase was 3.0 mM tetrabutylammoniumhydrogen sulfate (TBAHS) in ACN/water/triethylamine (TEA), (14, 85.6, 6.4 V/V) adjusted to pH 4.1 by glacial acetic acid. The detection was at 220 nm. The method was tested for linearity, accuracy, recovery, and specificity.
Keywords : HPLC, reverse phase, hydrochlorothiazide, enalapril maleate, stability, analysis