Determination of Ternary Mixtures of Vitamins (B1, B6, B12) by Zero-Crossing Derivative Spectrophotometry

Authors : Mahmure Üstün Özgür, Ikbal Koyuncu

Pages : 385-392

View : 11 | Download : 9

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :A new method for determining ternary mixtures of vitamin B1, B6 and B12 using second derivative spectrophotometry is described. The procedure is accurate, nondestructive and does not require any separation step or the solving of equations. Calibration graphs were linear up to 20 m gml-1 of vitamin B1 at 228.9 nm (r=0.9999), vitamin B6 at 309.6 nm (r=0.9999) and vitamin B12 at 361.7 nm (r=0.9998). The method was successfully applied for analyzing synthetic mixtures and commercial pharmaceutical preparations.
Keywords : Derivative spectrophotometry, Vitamins, B1, B6, B12.