Chromatographic Determination of Phenolic Acids in the Snowdrop by HPLC

Authors : Mustafa Tüzen

Pages : 49-54

View : 11 | Download : 8

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :Free and esterified phenolic acids of Galanthus elwesii (snowdrop) were extracted with petroleum ether and determined by HPLC. Cinnamic, ferulic, vanillic, p-coumaric, p-hydroxy benzoic, and caffeic acids were detected by a linear gradient elution with acetic acid/water (2:98 v/v) and acetic acid/acetonitrile/water (2:30:68 v/v) as the mobile phases in 30 min with a flow rate of 1.5 mL/min. Among the acids from snowdrop extracts, p-hydroxy benzoic was the major constituent followed by vanillic and ferulic acids.
Keywords : Galanthus elwesii (snowdrop), phenolic acids, HPLC