In vitro physiological effects of Origanum onites L. (Lamiaceae) essential oil treatment on human origin cell lines

Authors : Gul Ipek Gundogan, Ebru Ozdemir Nath

Pages : 158-171

View : 15 | Download : 9

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :Origanum onites L. belongs to the Lamiaceae family which is commonly used for symptomatic treatment of the diseases of the gastrointestinal, endocrine, and respiratory systems in Turkish ethnobotanical studies and public. In this study our aim was to investigate the physiological effects of O. onites essential oil (OE) on A549 human lung cancer, MIA PaCa human pancreas cancer and HUVEC human endothelial cell lines. Our findings showed that O. onites OE treatment at all concentrations (4.79-191.6 mu g/mL, 24 h) can inhibit cell proliferation and induce apoptosis in A549 and MIA PaCa cell lines (P = 38.32 mu g/mL) significantly inhibited cell proliferation. Apoptotic cell counts were significantly (P < 0.0001) higher in all cell lines at both incubation times (24 h 56.3 +/- 7%, 48 h 53 +/- 5% for A549, 24 h 44 +/- 7%, 48 h 44.7 +/- 7% for MIA PaCa, 24 h 48.7 +/- 6%, 48 h 49.3 +/- 5% for HUVEC). Migration rates were decreased significantly (P < 0.0001) (24 h 64 +/- 6%, 48 h 67.2 +/- 4% for A549, 24 h 49 +/- 5%, 48 h 69.35 +/- 5% for MIA PaCa, 24 h 47 +/- 5%, 48 h 69.6 +/- 2% for HUVEC). Based on these results, OE has been shown to have promising anticancer effects.
Keywords : Origanum onites, cellular-migration, apoptosis, proliferation, cancer, wound healing