Biochemical and morphological responses to cadmium-induced oxidative stress in Cladophora glomerata

Authors : Abuzer Çelekli, Hilal Bulut

Pages : 222-231

Doi:10.3906/bot-2001-12

View : 18 | Download : 8

Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :This study aimed to assess the effect of cadmium Cd2+ concentrations on biovolume, pigments, malondialdehyde MDA , hydrogen peroxide, proline, total phenolic compounds, total protein, and total carbohydrate contents of Cladophora glomerata . Cultivations of the alga 8.0 ± 0.1 g as fresh weight were exposed to 7.5, 15, 30, and 60 mg/L of Cd2+ ions in 500 mL flasks containing 400 mL of the medium on a shaker at 120 rpm for 7 days. Results of Fourier transform infrared analyses indicated that the amide, anionic, and amino groups had significant rolesin the binding of Cd2+ on C. glomerata. The detrimental effects of the Cd2+ dose not only observed the morphology of the algal cell but also changed the biochemical compounds of C. glomerata. Growth gradually decreased when the alga was exposed to Cd2+ at 15 and 60 mg/L in comparison with the control. High Cd2+ ions concentrations decreased in chlorophyll-a from 14.27 mg/g in control to 9.97 mg/g at 60 mg/L Cd and protein content from 43.60 mg/g in control to 21.66 mg/g at 60 mg/L Cd in the treated cells compared to the control group, whereas they increased stress molecules e.g., MDA and proline as biomarkers in the response mechanisms of algae to Cd2+ exposure. Results indicated that this alga had wide tolerance to high cadmium concentrations, and the stress compounds in the alga with exposure of Cd2+ seemed to be parameters as a biomarker for metal-induced oxidative stress.
Keywords : Biochemical response, cadmium, Cladophora glomerata, pigmentation