Genetic characterization of Orobanche cumana populations from the Thrace region of Turkey using microsatellite markers

Authors : Behiye Banu Bilgen, Ahmet Kubilay Barut, Sefer Demirbaş

Pages : 38-47

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Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :Orobanche cumana is a parasitic plant that can lead to advanced losses in yield of agricultural lands cultivating sunflower, depending on the sunflower varieties and the level of contamination. In our study, genetic diversity of six O. cumana populations from the Thrace region of Turkey was determined with the help of eight SSR (microsatellite) loci. All SSR loci were polymorphic. A total of 23 alleles were determined for the analyzed 120 samples. Allele number of each SSR locus ranged from two to six. Mean number of alleles (N a = 2.271), effective allele number (N e = 1.667), Shannon?s information index (I = 0.547), and heterozygosity levels (H o = 0.207 and H e = 0.340) were calculated. A high proportion of the genetic variation (66%) was due to within-population variation and 34% of the diversity was due to among-population variations. Based on the UPGMA dendrogram and STRUCTURE analysis, there were two main clusters. Cluster I was classified into three groups containing four populations from the Kırklareli and Edirne regions. Two populations from Tekirdağ were in cluster II. The information obtained from this study is valuable to provide a significant contribution to studies on the genetic structure, diversity, and race evolution of O. cumana and to the developed crop breeding studies and management strategies for controlling O. cumana infestation.
Keywords : Broomrape, Helianthus annuus, molecular marker analysis, simple sequence repeats