Regeneration and Agrobacterium-mediated Transformation Studies in Tomato ( Lycopersicon esculentum Miller)

Authors : Hüseyin Avni Öktem, Yeter Bülbül, Emre Öktem, Meral Yücel

Pages : 345-348

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Publication Date : 9999-12-31

Article Type : Makaleler

Abstract :An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. This work summarizes a simple culture method to obtain rooted plants from leaf pieces of two tomato cul-tivars (ES58 and WC156) within 3-4 months. Here, we also present our preliminary results on transformation experiments carried out with 8-day-old cotyledon explants and Agrobacterium tumefaciensEHA105 strain.
Keywords : Tomato, regeneration, Agrobacterium, transformation, Lycopersicon