Age-related Changes in Adhesive Phenotype of Bone Marrow-derived Mesenchymal Stem Cells on Extracellular Matrix Proteins

Authors : Kamelia Hristova-panusheva, Milena Keremidarska-markova, George Altankov, Natalia Krasteva

Pages : 11-19

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Publication Date : 2017-04-30

Article Type : Research

Abstract :Mesenchymal stem cells (MSCs) are a promising cell source for cell-based therapies because of their self-renewal and multi-lineage differentiation potential. Unlike embryonic stem cells adult stem cells are subject of aging processes and the concomitant decline in their function. Age-related changes in MSCs have to be well understood in order to develop clinical techniques and therapeutics based on these cells. In  this work we have studied the effect of aging on adhesive behaviour of bone marrow-derived MSC and MG- 63 osteoblastic cells onto three extracellular matrix proteins: fibronectin (FN), vitronectin (VN) and collagen  I (Coll I). The results revealed substantial differences in adhesive behaviour of both cell types during 21 days in culture. Bone-marrow derived MSCs decreased significantly their adhesive affinity to all studied proteins after 7th day in culture with further incubation. In contrast, MG-63 cells, demonstrated a stable cell adhesive phenotype with high affinity to FN and Coll I and low affinity to vitronectin over the whole culture period. These data suggest that adhesive behaviour of MSCs to matrix proteins is affected by aging processes unlike MG-63 cells and the age-related changes have to be considered when expanding adult stem cells for clinical applications.
Keywords : cell morphology, cell attachment and spreading, fibronectin, vitronectin, collagen I