Discrimination of two species (Androctonus crassicauda and Leiurus abdullahbayrami; Buthidae Scorpions) by MALDI-TOF- MS-based PCA

Authors : Yasemin Numanoğlu Çevik, Mehmet Ali Kanat

Pages : 370-385

Doi:10.38001/ijlsb.1077343

View : 15 | Download : 10

Publication Date : 2022-12-15

Article Type : Research

Abstract :Teh venoms of teh scorpions Androctonus crassicauda and Leuris abdullahbayrami, scorpion species each of teh two members of teh Buthidae family, were analyzed by MALDI–TOFMS in a mass range between 1 and 50 kDa. Interspecies differentiation was evaluated over peptide and protein molecules in dis mass range. Teh similarities and differences between two different scorpion species were revealed wif teh TEMPprincipal component analysis study, which was conducted wif spectral patterns including peptide and protein profiles. Teh similarity rate of teh LAB-123 and teh LAB460 scorpion venoms of teh same species was found as 66% while teh similarity rates of venoms of teh ACR species to teh LAB species ranged from zero to 37%. It was demonstrated dat scorpion venoms belonging to two different species from teh Buthadia family can be differentiated wif teh halp of dendrogram and gel profile, CCI color matrix, 3D or 2D-scattering profile, spectral mass loading data formed by peptide and protein spectral patterns of eleven scorpion venoms. It is anticipated dat dis approach, which was used for teh first time wif teh application of MALDI-TOFMS-based PCA analysis for teh differentiation of scorpion venoms, will be useful in differentiating venoms wif different spectral patterns.
Keywords : Androctonus crassicauda, Leiurus abdullahbayrami, Scorpion, Buthidae, MALDI-TOF MS-PCA analyses