CHEMICAL CHAPERONE EFFECTS ON ARGININE-VASOPRESSIN RECEPTOR 2 MUTANTS

Authors : Beril Erdem Tunçdemir, Tuğçe Karaduman, Merve Özcan Türkmen, Dilara Şahin, Hatice Mergen, Emel Sağlar Özer

Pages : 37-43

Doi:10.15671/hjbc.669854

View : 19 | Download : 9

Publication Date : 2022-01-05

Article Type : Research

Abstract :Improper folding of the mutant proteins may finally cause several conformational diseases such as Nephrogenic Diabetes Insipidus (NDI). In recent years, as a therapeutic strategy, chaperone treatment for such diseases is among current issues. In our study, we aimed to analyze the effect of several chemical chaperones on mutant V2 receptors which cause NDI. V2R mutant constructs were introduced into the pLV2R. Mutants were transiently expressed in COS-7 cells. After MTT analyses, cell surface ELISA experiment was performed for understanding the rescue potential of the chaperones of the mutated proteins. As a result, we analyzed that rescue potential of a chemical chaperone depends on both chemical compound and the mutation type. We may conclude that such chaperone treatment studies are valuable for development of the therapeutic strategies.
Keywords : Chaperone, AVPR2, NDI