Preparation of bacterial cellulose/vinyl imidazole-based membranes for selective purification of Hemoglobin

Authors : Monireh Bakhshpour, Adil Denizli

Pages : 423-431

Doi:10.15671/hjbc.921540

View : 18 | Download : 8

Publication Date : 2021-05-25

Article Type : Research

Abstract :The development of new biomaterials is needed to use in many areas such as protein purification, removal and drug release system. In this study, we fabricated hemoglobin (Hb) surface imprinting onto the bacterial cellulose nanofibers. The metal ions coordination was obtained with vinyl imidazole and Ni2+ ions. The selective purification of Hb was obtained using Hb-imprinted bacterial cellulose. The Scanning Electron Microscopy, Surface area, Transformed Infrared Spectroscopy, swelling tests and contact angle measurements were used for the characterization of Hb-imprinted and non-imprinted bacterial cellulose membranes. In addition, the adsorption studies were experimented with using the Hb aqueous solution in the batch systems at 2 h. In this study, the prepared bacterial cellulose was reported unique biomaterials for selective and sensitive purification of Hb with high adsorption capacity. The reusability of bacterial celluloses was demonstrated during the experiment.
Keywords : Purification, ions coordination, bacterial cellulose, hemoglobin.