In Vitro Shoot Regeneration of Oregano Origanum minutiflorum O. Schwarz & Davis

Authors : Dudu Özkum

Pages : 97-100

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Publication Date : 2007-04-01

Article Type : Other

Abstract :In vitro shoot regeneration procedure for oregano O. minutiflorum O. Schwarz & Davis , endemic species of Turkey,is described. Leaf segments and shoot explants hypocotyl, single nodal segment and shoot tip excised from 30-40d old in vitro germinated seedlings and cultured on Murashige and Skoog MS and Gamborg B5 media containingdifferent combinations of benzyl amino purine BAP 0.0, 1.0, 2.0 or 3.0 mg L-1 and naphtalene acetic acid NAA 0.0, 0.1 or 0.5 mg L-1 . The single nodal segments were the most succesful explant in all hormone combinationsused. MS medium supplemented with 2.0 mg. L-1BAP + 0.1 mg L-1NAA was the most effective medium for shootformation. However for all explants, B5 medium with all phytohormone combinations had no effective success andtherefore were not suitable on in vitro shoot regeneration
Keywords : Origanum minutiflorum, shoot regeneration, In vitro, micropropagation