An Antioxidant Combination Improves Histopathological Alterations and Biochemical Parameters in D-Galactosamine-Induced Hepatotoxicity in Rats

Authors : Tunc Catal, Sevim Tunali, Sehnaz Bolkent, Refiye Yanardag

Pages : 14-19

Doi:10.5152/EurJBiol.2017.1703

View : 21 | Download : 12

Publication Date : 2017-06-01

Article Type : Research

Abstract :The protective effects of a combination of antioxidants on histopathological changes and biochemical parameters were examined in D-galactosamine (D-GaIN)-induced hepatotoxicity in rats. Physiological saline solution was injected intraperitoneally into the control group, while D-GaIN (500 mg kg-1) was administered intraperitoneally into the experimental animals. The combination of 100 mg/kg/day ascorbic acid, 100 mg/kg/day alpha tocopherol, 15 mg/kg/day beta carotene, and 0.2 mg/kg/day sodium selenate was administered orally to intact control rats for 3 days. The same antioxidant combination was given to the D-GaIN group. Liver and blood samples were used for histopathological and biochemical assays. Liver tissues were significantly damaged by D-GaIN administration based on the histopathological findings. Serum aspartate and alanine transaminase, gamma glutamyl transpeptidase, alkaline phosphatase, lactate dehydrogenase, sialic acid, and uric acid levels increased. Serum and liver glutathione levels decreased, and serum superoxide dismutase, glutathione peroxidase, and glutathione-S-transferase, catalase activities as well as lipid peroxidation values increased in the D-GaIN group. In contrast, administration of the antioxidants reversed the histopathological and biochemical changes in the liver of animals administered D-GaIN. In conclusion, the administration of a combination of antioxidants suppressed histopathological changes and biochemical parameters in rats given D-GaIN.
Keywords : Vitamin C, β-carotene, D-galactosamine, sodium selenate, vitamin E, liver damage