Development and Evaluation of Epigenetic Regulation of Sucrose Metabolism in Saccharomyces cerevisiae by Using COBRA Technique and Selected CpG Islands in HXT10 and SUC2 genes

Authors : Burak Alaylar, Medine Güllüce, Mehmet Karadayi

Pages : 50-55

View : 19 | Download : 10

Publication Date : 2019-06-27

Article Type : Research

Abstract :Epigenetics mostly focuses on heritable changes in gene expression that does not contain changes to the underlying primary DNA sequences; an alteration in phenotype without an alteration in genotype. Among various cellular mechanisms, especially DNA methylation plays a central role in many epigenetic processes. Therefore, it has been the most studied epigenetic mechanism and many special techniques for assessment of changes in DNA methylation patterns have been developed. Combined bisulfite restriction analysis (COBRA) is one of these commonly used techniques. Although many studies have been conducted to get information about epigenetic regulation mechanisms in mammalian cell by using COBRA, there is still limited information on the the epigenetic programs and related pathways in other organisms.  
Keywords : COBRA, DNA methylation