Identification and Characterization of Some Streptomyces Species Isolated from Symtomatic Potatoes in Erzurum Province of Turkey

Authors : Kenan Karagöz, Recep Kotan

Pages : 27-37

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Publication Date : 2017-04-30

Article Type : Other

Abstract :Potato common scab is a significant disease that reduces the quality of potato tubers in worldwide. The goal of this study was to identify and characterize Streptomyces species, isolated scab lesions in potato production fields of Erzurum province, one of the important potato production areas of Turkey. Potato tubers with scab symptoms were collected from potato production fields, isolations were made and pathogenicity tests were carried out. Strains with positive pathogenicity reactions were identified according to classical and molecular methods. Polymerase chain reactions (PCR) were carried out with specific primers to assess the presence of TxtAB, Nec1, and TomA, genes considered common to the pathogenicity island (PAI) of Streptomyces sp. 47 of pathogenic strains were determined as S. scabiei , 15 strains as S. bottropensis , eight strains as S. stelliscabiei and four strains as S. europoeiascabiei. The species, S. stelliscabiei , S. bottropensis and S. europaeiscabiei heretofore had not been reported as pathogens of potato in Turkey. In addition, all identified strains are reported for the first time as present in the Eastern Anatolia Region of Turkey. Our testes revealed that the isolates mostly have the Nec1 , TomA genes and TxtAB but Some of the isolated strains have lacking of the three genes, based on our PCR tests.
Keywords : Scab, Streptomyces, Turkey, PCR, PAI