THE INVESTIGATION OF IN VITRO CALLUS FORMATION, REGENERATION AND MICROPROPAGATION OF DEVEGULU HOLLYHOCK, A MEDICINAL PLANT

Authors : Gül Görmez, Abdulhamit Battal, Musa Türker

Pages : 111-123

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Publication Date : 2019-12-27

Article Type : Research

Abstract :Devegülü hollyhock is used by public for the treatment of certain diseases. This study investigated the appropriate protocols for in vitro callus formation, regeneration and micropropagation of various explants of 14-day and 28-day old Devegülü seedlings germinated from the seeds in a sterile environment. Different concentrations of 2,4- dichlorophenoxy acetic acid (2,4-D) were used as plant growth regulator and Murashige and Skoog basal medium was used as basic medium. Regenerated plantlets were firstly transferred to rooting medium to promote root formation, after that they were planted into soil. Both 14-day and 28-day explants produced a higher rate of callus in MS medium including 2,4-D auxin hormone. The regenerated plantlets were obtained from the shoot tip and node explants of 28 days old seedlings after incubation in MS medium for 6 weeks. Direct regeneration from the node and shoot tip was observed in this hormone free medium. Micropropagation rates were 44.4 % and 12.5 % for node and shoot tip explants, respectively. With this study, callus formation, regeneration and micropropagation of the shoot tip and nodes was successfully carried for the first time.
Keywords : Devegülü, In Vitro Regeneration, Callus, Hollyhock