EVALUATION OF ANTIBACTERIAL EFFECT OF TOOTHPASTES WITH DIFFERENT CONTENTS: AN IN VITRO STUDY

Authors : Ayça Kurt

Pages : 13-22

View : 24 | Download : 11

Publication Date : 2017-10-02

Article Type : Research

Abstract :Objectives: Antibacterial substances added to different toothpastes that are presented to the market with the claim of showing antibacterial effect are aimed at preventing periodontal diseases and tooth decay by controlling pathogen formation in the bacterial plaque. The aim of this in vitro study is to evaluate toothpastes with different contents for their antimicrobial activity against Streptococcus Mutans. Materials and methods: Disc diffusion method was used to investigate the antibacterial effect of 11 toothpastes with different contents and different antibacterial agents (ozone, ganoderma lucidum, tea tree oil, xylitol, zinc, propolis, theobromine, triclosan, sodium lauril sarkosinat) against S. mutans. 0.1 mL sample of toothpaste was placed in the centers of paper discs with syringe. Vaseline was used as control group. 3 discs were prepared for each test product. The inhibition zone diameter against the microorganism was measured after 48 hours with digital calipers. Statistical analysis was performed by One-way ANOVA and posthoc TUKEY test. Results: In this study, it was found that Triclosan-containing toothpaste showed the highest antibacterial effect followed by toothpastes containing Ganoderma Lucidium and Xylitol respectively. Toothpastes containing Tea Tree Oil, Theobromine and Sodium Lauryl Sarkosinate were found with the similar antibacterial effects and had lowest efficacy among other antibacterial agents. Among 3 different toothpastes containing theobromine, no antimicrobial effect was observed in the toothpaste produced for children. Conclusion: In recent years, manufacturers have been trying to use new products while using natural ingredients as well as increasing their success targets with multiple effects. However, the efficiency of antibacterial agents used for this purpose is controversial. 
Keywords : Antibacterial effect, fluoride, Streptococcus mutans, toothpaste, zone of inhibition