Effect of Central and Peripheral Injected Nesfatin-1 on Electrocardiography in Rats

Authors : Kubra Ciftci, Gokcen Guvenc, Aysenur Bas, Murat Yalcin

Pages : 10-17

Doi:10.30782/uluvfd.445316

View : 30 | Download : 10

Publication Date : 2019-07-18

Article Type : Research

Abstract :Nesfatin-1 is an anorexic nucleobindin-2 -derived peptide and it has directly and centrally effect on the heart. The current study was designed to determine the effect of centrally and peripherally administered nesfatin-1 on electrocardiography (ECG) of healthy both fasted rats for 12 h and satiated rats fed ad libitum. In order to record ECG, the electrodes were placed limbs of at lead II under ketamine (50 mg/kg; im) and xylazine (20 mg/kg; im) anesthesia mix. Centrally administered different doses of nesfatin-1 (100 and 200 pmol; icv) resulted in dose- and time-dependently a statistically significant increase (p <0.05) in T wave, Q-T interval, and R-R interval duration without changing in ECG waves’ amplitude in both satiated and fasted rats. In a similar way, peripheral administration of nesfatin-1 (80 μg/kg; iv) in satiated rats prolonged statistically significant (p <0.05) T wave, Q-T interval, and R-R interval without producing a change in ECG waves’ amplitude. Moreover, icv administered nesfatin-1 in fasted and satiated rats, and iv injected nesfatin-1 in satiated rats induced a statistically significant decrease in heart rate (p <0.05). In conclusion, our findings suggest that centrally and peripherally administrated nesfatin-1 caused a delay in T wave, Q-T interval and two R-waves interval duration in ECG so that leading to a bradycardic effect in heart rate.
Keywords : Nesfatin-1, Electrocardiography, Intracerebroventricular, Intravenous, Heart rate