The role of nitrosative and oxidative stress in rainbow trout (Oncorhynchus mykiss) liver tissue applied mercury chloride (HgCl2)

Authors : Mehmet Reşit Taysi, Muammer Kirici, Mahinur Kirici, Hasan Ulusal, Bünyamin Sögüt, Seyithan Taysi

Pages : 269-273

Doi:10.12714/egejfas.38.3.02

View : 27 | Download : 6

Publication Date : 2021-09-15

Article Type : Research

Abstract :The aim of this study was to determine oxidative stress caused by mercury chloride (HgCl 2 ) in rainbow trout (Oncorhynchus mykiss ) liver tissue. For this purpose, the LD 50 value of HgCl 2 on rainbow trout was determined as 551 μg/L. In the study, 40 fish in four groups were exposed to 25% and 50% (138 and 276 µg/L) of the two subletal doses of HgCl 2 for 2 and 7 days, with 10 fish (n=10) in each group. To determine oxidative stress; peroxynitrite (ONOO−), total oxidant level (TOS), total antioxidant level (TAS), oxidative stress index (OSI) and malondialdehyde (MDA) were analyzed. In the study, it was observed that the differences between the groups in terms of ONOO−, TOS, TAS and OSI levels in the liver tissues was significant (P<0.05), however, this difference was not significant (P>0.05) in terms of MDA values. As a result, it can be concluded that HgCl 2 increases ONOO−, TOS, TAS, OSI and MDA levels in liver tissue and even small doses of mercury are toxic to fish. 
Keywords : Oncorhynchus mykiss, mercury toxicity, oxidant/antioxidant, peroxynitrite, liver