Kaldirik(Trachystemon orientalis) bitkisi Polifenol oksidaz enzimi üzerine metallerin etkisi

Authors : Esma Hande Alici, Gülnur Arabaci

Pages : 399-405

Doi:10.16984/saufbed.99926

View : 18 | Download : 7

Publication Date : 2013-06-01

Article Type : Other

Abstract :In this study, Polyphenol oxidase was extracted from Borage plant (Trachystemon orientalis) and the crude extract was used for the assays. Its pH and temperature optima for 4-methyl cathecol were 5.0 and 5°C, respectively. Km of this enzyme was 4.55 mM for 4-methyl cathecol. We also found that the enzyme was activated by Fe3+, Mg2+, Zn2+, Cu2+, Ca2+, K+, but inhibited by Hg2+, Mn2+, Ni2+, Sn2+, Na+. Ba2+, Al3+ and Pb2+ metal ions both activated and inhibited the enzyme at different concentrations.
Keywords : Trachystemon orientalis, Polifenol oksidaz, metal etkisi, enzim kinetiği