Response Surface Methodology for Optimization of Antagonist Effect of Bacillus sp.

Authors : Ecren Uzun Yaylacı

Pages : 126-132

Doi:10.53501/rteufemud.1448254

View : 34 | Download : 42

Publication Date : 2024-06-30

Article Type : Research

Abstract :The study aimed to optimize bacterial culture conditions to enhance the antagonist effect of B. pumilus PJ_11 on V. vulnificus. The experiment designed by the Box Behnken method showed that the antagonistic effect was highest (26.44 mm) at 30°C, NaCl concentration 2%, and 18 hours. According to the coefficient values of the regression equation, temperature was more effective on the response than other parameters. The combined effect of two variables, temperature and NaCl concentration, was more effective than other combinations. The results showed that the Box-Behnken design was an adequate method to optimize three factors of culture conditions.
Keywords : Bacillus, Cevap Yüzeyi Yöntemi, Antagonistik etki