- Hayvan Bilimi ve Ürünleri Dergisi
- Vol: 6 Issue: 1
- The Expression Dynamics of Key Immune-Related Genes in Response to Mannheimia Haemolytica in Sheep A...
The Expression Dynamics of Key Immune-Related Genes in Response to Mannheimia Haemolytica in Sheep Alveolar Macrophages In Vitro
Authors : Saif Adil Abbood Al-janabi, Mehmet Ulaş Çinar, Ghulam Asghar Sajid, Ahmed Qasim Naji Naji, Md Mahmodul Hasan Sohel
Pages : 1-13
Doi:10.51970/jasp.1255946
View : 23 | Download : 22
Publication Date : 2023-06-29
Article Type : Research Article
Abstract :Alveolar macrophages (AMs) respond to these infections as the first line of defense and trigger the lung\'s immune response. Knowing the expression dynamics of key immune-related genes in alveolar macrophages against M. haemolytica challenge will help deepen the understanding of disease immunopathogenesis. For this purpose, first time an in vitro obtained from bronchoalveolar lavage (BAL) fluid and treated with three doses (T1: 1800, T2: 2700, T3: 5400 CFU) of M. haemolytica inoculum, while keeping one untreated as a control. Then, total RNA was extracted, and cDNA was synthesized. The real-time quantification data indicated that the expression of IL-1β, IL-6, IL-8, IL-10, and NF-κB genes were significantly changed as compared to control. Our study revealed that the exposure to M. haemolytica stimulates the immune response in the sheep alveolar macrophages in a dose and time-dependent manner.Keywords : Alveolar makrofaj, Bağışıklıkla ilişkili genler, Gen ifadesi, M. haemolytica, pnömoni