- Harran Üniversitesi Veteriner Fakültesi Dergisi
- Vol: 10 Issue: 2
- Identification of Vibrio parahaemolyticus by PCR Method in Fish and Water Samples from Tigris River,...
Identification of Vibrio parahaemolyticus by PCR Method in Fish and Water Samples from Tigris River, Turkey
Authors : Filiz Özcan, Neval Berrin Arserim, Aydın Ketani
Pages : 191-195
Doi:10.31196/huvfd.1009028
View : 23 | Download : 9
Publication Date : 2021-12-12
Article Type : Research
Abstract :Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen. Water temperature is an important ecological factor. It is consequently of high importance to follow V. parahaemolyticus in aquatic environments and foods. The aim of this work was to detect the presence of V. parahaemolyticus in Tigris River from eight different sites namely Malabadi Bridge, Fiskaya, Ongözlü Bridge, Bağıvar, Pamukçay, Ambarçay, Seyrantepe and Silvan. Total 480 samples of water and fish were used for V. parahaemolyticus detection. Thiosulphatecitrate‐bile salt sucrose (TCBS) Agar was employed for bacteria isolation for 24 h at 37◦C. The dominant uniform bacterial colonies were purified by streaking onto the TCBS agar plates three times. Identification of this pathogen was made by PCR. A total of 480 water and fish samples were analyzed. According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samples taken from the Seyrantepe region.Keywords : pcr, Vibrio parahaemolyticus, Fish, Water