- Celal Bayar Üniversitesi Fen Bilimleri Dergisi
- Vol: 14 Issue: 4
- Exogenous Polyamine Treatment Prevents UV-A Induced Cellular Damage and Photoinhibition of Photosyst...
Exogenous Polyamine Treatment Prevents UV-A Induced Cellular Damage and Photoinhibition of Photosystem II in Lichen Physcia semipinnata (J. F. Gmel.) Moberg
Authors : Dilek Ünal, Inci Tüney Kizilkaya
Pages : 399-404
Doi:10.18466/cbayarfbe.410546
View : 29 | Download : 10
Publication Date : 2018-12-28
Article Type : Research
Abstract :In this study the protection ability of polyamines on Photosystem II (PSII), the proline content of the cells and necrosis formation induced by Ultraviolet-A irradiation were studied. Although 24 h Ultraviolet-A exposure decreased the photosynthetic quantum yield (Fv/Fm) ratio in Physcia semipinnata (J. F. Gmel.) Moberg, thalli that treated with 1 mM polyamine, especially spermidine and spermine, were slightly influenced by the UV-A exposure. It was also found that spd and spm treated samples had lower proline content than putrescine (put)-treated samples. Moreover, It was found that the samples that were treated with spd and spm had lower percentage of DNA damage than put-treated samples and non-treated groups. In this study, Ultraviolet-A induced DNA damage detected by acridine orange/ethidium bromide staining assay. The DNA damage in thalli of P. semipinnata was detected in situ in phycobiont layer after 24 h of Ultraviolet-A exposure, in contrast, polyamine-treated samples had a lower rate of necrotic cells.Keywords : polyamine, photosystem II, proline, UV-A, lichen