- Bor Dergisi
- Vol: 7 Issue: 2
- The effect of boric acid on copine-7 expression and bioactivity in dental pulp-obtained stem cells
The effect of boric acid on copine-7 expression and bioactivity in dental pulp-obtained stem cells
Authors : Serife Buket Bozkurt, Sema Sezgin Hakki
Pages : 461-466
Doi:10.30728/boron.1069145
View : 17 | Download : 6
Publication Date : 2022-06-30
Article Type : Research
Abstract :Boron has significant impact on the mineral composition of teeth. Copine-7 (Cpne-7) is secreted by pre-ameloblasts and induces dentin formation via differentiation of mesenchymal cells of dental. The goal of this study was to see how boric acid affected the bioactivity and expression of Cpne-7, collagen type I in dental pulp-obtained stem cell. The expression of Cpne-7, COL-I were assessed in the boric acid treated pulp cells by molecular method on 3 and 8 days exposure. When comparing the different boric acid concentrations to the control group in a proliferation experiment, no significant differences were noted. At 10 ng/mL boric acid, a rise in the number of mineralized nodules was observed. Boric acid concentrations (1 ng/mL, 10 ng/mL) increased the transcripts of Cpne-7 on day 3 and 8. Additionally, 1 ng/mL boric acid concentration significantly upregulated of Cpne-7 expression compared to control group on day 3 and 8. When the 1-10 ng boric acid was compared to control group COL-I expression remarkably enhanced in cell. According to the current findings that boric acid may be a potent regulator of Cpne-7, which is a promising candidate for new dentin formation in regenerative dentistry, in dental pulp-obtained mesenchymal stem cells and provide osteogenic efficacy in therapies aimed at dentin formation.Keywords : Boric acid, Cpne-7, Pulp mesenchymal stem cells