- Pediatric Practice and Research
- Cilt: 11 Sayı: 3
- The Effect of Topiramate on Ischemia-Modified Albumin and Prolidase Enzyme Levels in Nitroglycerin-I...
The Effect of Topiramate on Ischemia-Modified Albumin and Prolidase Enzyme Levels in Nitroglycerin-Induced Adolescent Rat Brain Tissue
Authors : Yılmaz Koçak, Nesrin Ceylan, Maşallah Ermaya, Halit Demir, Yıldıray Basbugan
Pages : 153-157
View : 148 | Download : 168
Publication Date : 2023-10-15
Article Type : Research
Abstract :Aim: Migraine is a neurological disorder accompanied by episodic headaches that can also occur in children and adolescents. Environmental and genetic factors play a role in the pathogenesis of migraine and the possible contribution of oxidative stress (OS) is debated. Therefore, the present study focused on the possible effect of topiramate (TPM) on the oxidative stress markers ischemia-modified albumin (IMA) and prolidase enzyme activity in nitroglycerin (NTG)-induced brain tissue of adolescent rats. Material and Method: In this study, 32 male Wistar albino rats aged 6 weeks (adolescence) were used. Rats were divided into four groups with 8 animals in each group. Rats were administered NTG intraperitoneally (i.p.) once daily and topiramate (TPM) orally (p.o.) twice daily. Groups and doses were formed as follows. Group 1: Control group; Group 2: NTG (10 mg/kg) group, Group 3: NTG (10 mg/kg) + TPM (50 mg/kg) group and Group 4: Only TPM (50 mg/kg) group. Animals were sacrificed at the end of the experiment. After the brain tissues were homogenized, IMA and prolidase enzyme activities were measured spectrophotometrically. Result: IMA and prolidase levels increased in the NTG-treated group and this increase was found to be significant when compared with the control group (p≤0.05). The combination of NTG+TPM brought IMA and prolidase levels closer to the control group and numerically reduced them compared to the NTG group. Only the TPM group showed a significant decrease compared to the NTG group (p≤0.05). In addition, IMA and prolidase levels of TPM were close to the control group. Conclusion: NTG increased IMA and prolidase levels, indicating that it may trigger oxidative stress. TPM partially reversed IMA and prolidase levels. These results may support the hypothesis that TPM may have an antioxidant effect that suppresses OS. Understanding the pharmacodynamic effects of TPM may enable its more effective use as a therapeutic agent.Keywords : Topiramat, Nitrogliserin, IMA, Prolidaz, Adolesan rat, Oksidatif stres