- Natural and Engineering Sciences
- Vol: 7 Issue: 1
- In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium
In Vitro Micropropagation of Pogostemon erectus (Dalzell) Kuntze in Liquid Culture Medium
Authors : Muhammet Dogan
Pages : 80-88
Doi:10.28978/nesciences.1098681
View : 11 | Download : 3
Publication Date : 2022-04-04
Article Type : Research
Abstract :Pogostemon erectus (Dalzell) Kuntze is a valuable aquatic-ornamental plant. This study was designed for the multiplication of P. erectus in a liquid culture medium. In this context, shoot tip explants of P. erectus were placed in Murashige and Skoog (MS) food solutions including Zeatin (ZEA: 0.1-2.4 mg/L) and indole acetic acid (IAA: 1.2 mg/L). In addition, experiments were set up in hormone-free environments as controls. In general, high-frequency results were recorded. 100 % regeneration was determined in the treatment of 0.6 mg/L ZEA + 1.2 mg/L IAA. The best results for shoot count and shoot length were obtained with 0.6 mg/L ZEA + 1.2 mg/L IAA and 0.3 mg/L ZEA + 1.2 mg/L IAA, respectively. Generally, low regeneration values were seen in nutrient solutions using high or low doses of ZEA. Shoots were rooted in MS nutrient media with 0.25 mg/L IAA. Then they were transferred to an aquarium and successfully adapted to external conditions.Keywords : MS medium, Shoot regeneration, Shoot tip explant, Tissue culture